Efficient degradation of soybean protein B3 subunit in soy sauce by ultrasound-assisted prolyl endopeptidase and its primary mechanism

Zhankai Zhang,Pei Shan,Zhi-Hong Zhang,Ronghai He, Limin Xing, Jianbin Liu, Degui He,Haile Ma,Zejian Wang,Xianli Gao

FOOD CHEMISTRY（2023）

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摘要

•Prolyl endopeptidase reduced soy sauce secondary precipitate via degrading B3 subunit.•Ultrasound-assisted prolyl endopeptidase further reduced soy sauce secondary precipitate.•Ultrasound improved prolyl endopeptidase’s structure and thus enhanced its activity.•Degradation rate increase of B3 subunit is attributed to increase of the enzyme’s activity.

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关键词

Soy sauce,Ultrasound,Prolyl endopeptidase,Soybean protein,Aspergillus oryzae,Aspergillus niger

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