Effect of entecavir plus peginterferon a-2a on hepatitis B virus-RNA, IL-21 expression level, immune function and prognosis in patients with hepatitis B

Combination of interferon and nucleotide analogues might be more effective in inhibiting replication of hepati-tis B virus (HBV), thereby alleviating hepatic fibrosis and hepatocyte necrosis. Herein, we explored the impact of co-treatment of entecavir IP:(ETV) 203.8and 109.20 PEG-IFN ?-2a On: Sun, on 8 JunHBV-RNA, 2023 IL-2110:23:19level, immune function and prog-nosis in HBeAg-positive patients with low HBsAg level. Patients treated with ETV over 12 months received Copyright: Amercan Scientific Publishers PEG-IFNa-2a treatment. Serum HBV markers and HBV-DNA were examined at 12, 24 and 48 weeks, and Deivered by Inge ta disappearance of HBsAg were measured at 24 weeks as an evaluation index for efficacy. Besides, levels of IL-21, serum albumin, and complement C3 were measured. During follow-up, we found that, HBsAg serologi-cal conversion appeared in 6.0% of all patients, with HBsAg disappearance in 18.1% and HBsAg <10 IU/mL in 10.8%. The HBeAg level in non-disappearing group was lower than HBsAg disappearing group (P < 0.05) with AUC score of 0.720 for HBsAg suggested by HBeAg conversion rate (P < 0.05). Of note, HBsAg levels decreased at 12 weeks of treatment (& GE;0.5 log IU/mL) and HBsAg was prone to disappearance (P < 0.05). AUC of HBsAg disappearance predicted by the HBsAg levels at 12 weeks and 24 weeks were 0.810 (95% CI: 0.686-0.935, p = 0.000) and 0.842 (95% CI: 0.736-0.947, p = 0.000) compared with baseline. Combination of ETV and PEG-IFNa-2a resulted in enhancement of serum IL-21 level (210.803 & PLUSMN; 72.477) and reduction of IL-21 expression (157.084 & PLUSMN; 38.697). The IL-21 level was negatively correlated with immune function indi-cators serum albumin and serum complement C3. HBsAg conversion rate and early HBsAg changes are crucial to prediction of HBsAg disappearance. With ETV achieving great virological response, combination of ETV with PEG-IFNa-2a enhanced HBsAg loss, reducing IL-21 expression, and improving immune function in patients.