The Optimum Condition Design of Microwave-assisted Extraction Combined Lactic Acid-Sucrose Based Natural Deep Eutectic Solvent on Polyphenols Enrichment from Eleutherine bulbosa Mill. Urb. Bulbs

The advantages of natural deep eutectic solvent (NADES) for bioactive extraction to create formulations for nutraceuticals and cosmetics include biodegradability, biocompatibility, secondary metabolite extractability, and food grade. This study aims to design the optimum condition of microwave-assisted extraction (MAE) combined lactic acid-sucrose (LA-Suc)-based NADES on polyphenolic enrichment from the bulbs of Eleutherine bulbosa Mill. Urb. using response surface methodology (RSM). In the present study, the condition of MAE-combined LA-Suc-based NADES parameters, including NADES (LA-Suc) ratio, microwave irradiation time, solid-liquid ratio, and microwave irradiation power, had been involved. RSM with four factors and three levels (Box-Behnken Design) was applied to design a predictive model and optimize the MAE-combined LA-Suc-based NADES condition. The response surface was calculated using the total polyphenols content (TPC) value as the dependent variable. According to the result, the optimum condition of MAE combined LA-Suc based NADES was recommended according to RSM analysis as follows: NADES (LA-Suc) ratio of 4:1 g/g, irradiation time of 10 min, the solid-liquid ratio of 1:10 g/mL, and irradiation power of 360 Watts. The scale-up confirmation test using a 50 g sample (ten times the number of the sample) was observed to be 82.434 +/- 1.623 mg GAE/g sample.