Rapid Detection of Human Adenovirus by Loop-Mediated Isothermal Amplification of Nucleic Acids Extracted Using Magnetic Nanoparticle Enrichment Technology

The human adenovirus (HAdV) genotypes HAdV-3 and HAdV-7 are currently the most prevalent variants in China and are responsible for frequent mass outbreaks. Early diagnosis is crucial for infection control and timely treatment. Here we established an assay using loop-mediated isothermal amplification (LAMP) for rapid detection of nucleic acids extracted using magnetic nanoparticle enrichment technology to detect these two HAdV genotypes. The LAMP assay results were 100% consistent with those obtained using quantitative polymerase chain reaction, and the turnaround time was shorter, enabling earlier detection, diagnosis, and treatment of patients.