ENZYMATIC HYDROLYSIS OPTIMIZATION OF PRETREATED MUNICIPAL SOLID WASTE USING A MIXTURE OF ENZYMES PRODUCED BY ASPERGILLUS NIGER

This study aimed to check the potential of wheat bran and biodegradable municipal solid waste enzyme cocktail to produce reducing sugar of high yield from the pretreated substrate. The enzyme cocktails were produced from Aspergillus niger fermentation using wheat bran and biodegradable municipal solid waste as carbon sources. The results showed that these enzyme cocktails had beta-glucosidase and endoglucanase activities. The maximum yields of reducing sugar, i.e., 87% and 83%, were achieved at 10% substrate concentration, 50 degrees C temperature, and 36 h fermentation time using wheat bran and biodegradable municipal solid waste enzyme cocktail, respectively. Moreover, a comparison of batch and fed-batch processes of enzymatic hydrolysis at a high loading of substrate dose showed that, in the fed-batch process, an increase in the yield of reducing sugar (83%) was achieved, as compared to the batch process (73%) using WB enzyme cocktail.