W/O Pickering emulsion catalytic system stabilised by acetylated arachin/chitosan nanoparticles as an efficient platform for the preparation of diacylglycerol-rich soybean oil

Pickering emulsions stabilised by nanoparticles are recognised as enzyme carriers, enabling advanced catalysis efficiency. Herein, we immobilised lipase on acetylated arachin nanoparticles/chitosan composites (AAPs/CS) through cross linking with glutaraldehyde (GDA) resulting in the formation of AAPs/CS-Lipase to stabilise water in oil (W/O) Pickering emulsion catalytic systems (PEC). The specific activity of AAPs/CS-Lipase was increased to 160% compared with free lipase (1.48 & PLUSMN; 0.02 U mg(-1)). Increasing the mass of CS led to an increase in the droplet size of W/O PEC from 2.53 & PLUSMN; 0.82 & mu;m to 7.94 & PLUSMN; 1.24 & mu;m. AAPs/CS-Lipase decreased the interfacial tension and enhanced the interfacial viscosity of W/O PEC. The catalytic efficiency of W/O PEC is 2.75 times of biphasic catalytic system (BCS). The content of DAG exceeded 50% of the total oil. The specific activity of AAPs/CS-Lipase remained above 70% even after 10 cycles. W/O PEC provided an efficient catalytic platform to prepare DAG-rich soybean oil.