LINC02582 Sponges MiR-375 To Promote The Lung Metastasis Of Colorectal Cancer Via Lactate Dehydrogenase B Dependent Glycolysis

Abstract Glycolysis plays significant roles in reprogramming the metastatic tumor microenvironment. Many lncRNAs have been reported to function as oncogenes by regulating glycolysis. In the present study, we found that the expression of LINC02582 gradually increased from adjacent normal to colorectal cancer (CRC), especially in Stage IV tumor tissues, and high LINC02582 expression predicted poor survival, especially for patients with lung metastasis. Functionally, LINC02582 promoted the glycolysis and invasion of CRC cells in vitro and enhanced their lung metastasis in vivo. Mechanically, LINC02582 functioned as a ceRNA to regulate lactate dehydrogenase B (LDHB) expression via sponging miR-375, resulting in glycolysis of CRC cells and leading to their invasion and lung metastasis. Clinically, high LINC02582 and LDHB expression predicted poor survival of CRC patients. In conclusion, the present study identified LINC02582 as an onco-lncRNA in CRC and demonstrated that the combination of LINC02582 /miR375/LDHB may be a potential prognostic biomarker of CRC with lung metastasis.