MP34-03 THE ACCESSORY GENOME LANDSCAPE OF PROTEUS MIRABILIS

You have accessJournal of UrologyCME1 Apr 2023MP34-03 THE ACCESSORY GENOME LANDSCAPE OF PROTEUS MIRABILIS Robert Potter, Kailun Zhang, Ben Reimler, Jamie Marino, Carol Muenks, Kelly Alvarado, Meghan Wallace, Lars Westblade, Erin McElvania, Melanie Yarbrough, David Hunstad, Carey-Ann Burnham, and Gauatm Dantas Robert PotterRobert Potter More articles by this author , Kailun ZhangKailun Zhang More articles by this author , Ben ReimlerBen Reimler More articles by this author , Jamie MarinoJamie Marino More articles by this author , Carol MuenksCarol Muenks More articles by this author , Kelly AlvaradoKelly Alvarado More articles by this author , Meghan WallaceMeghan Wallace More articles by this author , Lars WestbladeLars Westblade More articles by this author , Erin McElvaniaErin McElvania More articles by this author , Melanie YarbroughMelanie Yarbrough More articles by this author , David HunstadDavid Hunstad More articles by this author , Carey-Ann BurnhamCarey-Ann Burnham More articles by this author , and Gauatm DantasGauatm Dantas More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000003268.03AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Proteus mirabilis is a Gram-negative bacterium recognized for its unique swarming motility, urease activity, and propensity to cause urinary tract infections. Early proteomic analysis on four strains indicated that, unlike other Gram-negative bacteria, P. mirabilis may not exhibit significant variation in gene content. However, there has not been a focused analysis of large numbers of P. mirabilis genomes from various sources. Our objective was to perform the first large scale genomic analysis of P. mirabilis to understand how bacterial diversity may impact if an isolate is recovered from a urinary or non urinary source. METHODS: We performed comprehensive genomic analysis on 2,060 Proteus genomes. We sequenced the genomes of 893 clinical isolates from three large US academic medical centers, and also included 1,006 genomes from NCBI Assembly and 161 genomes assembled from Illumina reads previously uploaded to NCBI SRA. We used average nucleotide identity to delineate species and subspecies, core genome phylogenetic analysis to identify clusters of highly related P. mirabilis genomes, and pan-genome annotation to identify genes of interest not present in the model P. mirabilis strain HI4320. RESULTS: Proteus is composed of 10 named species and 5 uncharacterized genomospecies. P. mirabilis can be subdivided into three subspecies; subspecies 1 represented 96.7% (1,822/1,883) of all genomes. The P. mirabilis pan-genome includes 15,399 genes outside of HI4320, and 34.3% (5,282/15,399) of these genes have no annotation. Subspecies 1 is composed of several highly related clonal groups. Prophages and genetic elements encoding putatively extracellular-facing proteins are associated with clonal groups. Uncharacterized outside of P. mirabilis HI4320 genes with homology to known virulence-associated operons can be identified within the pan-genome. CONCLUSIONS: Similar to other Gram-negative bacteria, P. mirabilis has a mosaic genome with a linkage between phylogenetic position and accessory genome content. Beyond what is represented in the model strain HI4320, P. mirabilis encodes a variety of genes that may impact host-microbe dynamics. The diverse, whole-genome characterized strain bank from this work can be used in conjunction with reverse genetic and infection models to better understand the impact of accessory genome content on bacterial physiology. Source of Funding: RFP received project support from Academy of Clinical Laboratory Physicians and Scientists Young Investigator Grant Program, and salary support from American Urologic Association Research Scholar Program © 2023 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 209Issue Supplement 4April 2023Page: e459 Advertisement Copyright & Permissions© 2023 by American Urological Association Education and Research, Inc.MetricsAuthor Information Robert Potter More articles by this author Kailun Zhang More articles by this author Ben Reimler More articles by this author Jamie Marino More articles by this author Carol Muenks More articles by this author Kelly Alvarado More articles by this author Meghan Wallace More articles by this author Lars Westblade More articles by this author Erin McElvania More articles by this author Melanie Yarbrough More articles by this author David Hunstad More articles by this author Carey-Ann Burnham More articles by this author Gauatm Dantas More articles by this author Expand All Advertisement PDF downloadLoading ...