Biofabricated Gold Nanoparticles with Antibacterial and Antibiofilm Activities Against Foodborne Bacterial Pathogens Using Pseudomonas Aeruginosa Metabolic Extract

Antimicrobial resistance is becoming an important issue for global wellness due to the widespread applications of antibiotics and chemical sanitizers. Therefore, it is necessary to investigate alternate approache for combating bacterial infections. One strategy is utilizing cutting-edge materials, such as gold nanoparticles (AuNPs). This investigation used Pseudomonas aeruginosa metabolic extract (ME), as reducing and stabilizing agent, to bio-manufacture of AuNPs. The extract's active gradient was utilized to reduce Au ions and transform them into Au o while stabilizing the produced nanoparticles using extract ingredients. Three samples, designated as 0.2 AuNPs@ME, 0.4 AuNPs@ME, and 0.6 AuNPs@ME, of AuNPs have been prepared by combining different amounts of gold salt. Based on the research's results, the biogenic synthetic AuNPs had crystalline structures and an average particle sizes of 48, 70, and 84 nm, respectively. The biofabricated AuNPs' antibacterial activity was also evaluated against foodborne pathogens, and their capacity to prevent biofilm formation was measured. The results showed that, 0.4 AuNPs@ME could suppress biofilm formation and had a robust bactericidal impact against certain bacterial species. This result is significant since the production of biofilms is a typical persistence strategy used by bacteria, rendering them resistant to antimicrobial treatments and challenging to eradicate. This study's findings imply that biofabricated AuNPs have significant antibacterial properties against foodborne pathogens and can be used as an efficient antimicrobial agent in food safety and sanitation applications. A particularly intriguing feature of AuNPs is their capacity to prevent the development of biofilms, which could prove the difficulties caused by foodborne bacteria.