Difficulties and artefacts in cryo-fixation of ovarian tissues for X-ray fluorescence analyses

Even though measurements in cryogenic conditions are the preferred choice for single-cell and plant analyses to reduce sample preparation artefacts, preserve the integrity of samples and prevent loss of some chemical elements, cryo-fixation of human or animal soft tissue for X-ray fluorescence analysis still remains challenging. Here we report, compare and discuss analyses of bovine ovarian tissues performed in cryogenic or room temperature conditions, the latter both chemically fixed or freeze-dried.