Dendrobium huoshanense stem polysaccharide ameliorates high fructose-induced hepatocyte lipid accumulation through decreasing miR-429 to upregulate RIP2
JOURNAL OF FUNCTIONAL FOODS(2023)
摘要
Dendrobium huoshanense is an edible medicinal herb. Its stem polysaccharide (DHP) can alleviate hepatic injury, but the underlying mechanism is not fully understood. Receptor-interacting protein 2 (RIP2) is an important regulator in metabolic diseases. Here, we found that high fructose decreased RIP2 expression, and increased inositol-requiring enzyme-1 & alpha; (IRE1 & alpha;) and spliced X-box binding protein 1 (XBP1s) expression in cultured he-patocytes along with lipid accumulation, which were attenuated by DHP. RIP2 overexpression blocked high fructose-induced upregulation of IRE1 & alpha; and XBP1s as well as hepatocyte lipid accumulation. RIP2 knockdown reversed amelioration effect of DHP on hepatocyte lipid accumulation. Furthermore, high fructose increased hepatocyte miR-429 expression targeting RIP2. MiR-429 inhibitor reversed high fructose-induced down -regulation of RIP2 and lipid accumulation. Notably, DHP reduced miR-429 expression and increased RIP2 expression in its amelioration of lipid accumulation in this cell model. These results suggested that DHP pro-tected against high fructose-induced hepatocyte lipid accumulation through downregulating miR-429 to upre-gulate RIP2 expression.
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关键词
hepatocyte lipid accumulation,fructose-induced
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