Mutation Accumulation under UV Radiation and Chemical Mutagen for Meliorated Biodiesel Production Using B. Amyloliquefaciens (MF 510169)

The cost-effective production of biodiesel is a major concern to address the issues related to climate change and energy security for protecting the economy of both non-developed and under-developed countries. The use of synergistically mutated bacteria for biodiesel production is an emerging and advantageous impendent. In the present investigation, B. amyloliquefaciens (MF 510169) strain has been mutated firstly with UV-C (254 nm for 320 S) which produces 3.93 % oil. Thus, the mutated strain is explored for chemical mutagen; ethyl methane sulphonate (EMS) at a concentration of 20 & mu;g/ mL adduces the highest oil i.e. 6.43 % to produce the highest quantity of biodiesel. Synergistically mutated strain's lipids are extracted and transesterified using sulphuric acid (1.5 %) under optimal reaction conditions yielding 75 % fatty acid methyl esters (FAME's). Produced biodiesel is physico-chemically characterized using different spectroscopic techniques. The as-synthesized biodiesel fuel properties are nearly 75 % matched with ASTM International Standards. The model synergistic mutated strain is stable in terms of lipid production for up to 40 days. The recycling pattern of agro-waste substrate imparts best performance for up to three revisions consistently. The antioxidant stability of the as-synthesized biodiesel tested up to 25 days confirms moderate stability upon the addition of synthetic antioxidants. Overall, the synergistically mutated strain's fatty acids are modulated well fashion as compared to wild type strain.