Domäneninsertion: Untersuchung zur Konstruktion schaltbarer Hybridproteine

Domain insertion engineering is used to combine the functionality of unrelated proteins by introducing receptor domains into effector proteins, yielding allosteric effectors. However, the identification of suitable insertion sites is still cumbersome. To dissect biophysical and statistical indicators of domain insertion tolerance in various effector proteins, we combined unbiased insertion screening with machine learning. Applying our screening pipeline to the transcription factor AraC and a light-responsive LOV domain, yielded potent opto-chemogenetically regulated derivatives.