Performing in vitro biological assays to evaluate the impact of electrochemotherapy treatments

Electrochemotherapy (ECT) treatment is now a routine technique for the treatment of cutaneous and subcutaneous tumors. To better understand the underlying mechanisms and effects of electrochemical stimuli on cells, performing biological experiments is particularly important. We propose to evaluate the impact of ECT treatments on cell permeabilization and cell viability. We present here a comparison between two viability assays - crystal violet staining and clonogenicity assays, on mammalian cells submitted to pulsed electric field used along the ECT protocols.