Multi-metal Nanozyme Properties for Colorimetric Peroxidase Reaction: Overview of an Applicable Method Validation for H₂O₂ Detection

Displacement of enzyme mimic nanomaterials has been developed due to disadvantages of natural enzymes. Peroxidase reaction could be used for H2O2 colorimetric evaluation in different media. We aim to develop a new method based on the best catalytic activity of metal Nanozyms. A method for H2O2 detection was carried out by TMB peroxidation of metal nanozymes. In this way peroxidase activity of Ag-nano zeolite Y, Cu-nano zeolite Y, Zn-nano zeolite Y and Ag, Cu, Zn-nano zeolite Y was evaluated by Central Composite Design. Total of 28.42% from three metals was exchanged in multi metal nano zeolite Y (mMy) with higher peroxidase- like activity. Nanozyme Km and Vm were evaluated 0.076 and 8.76 x 108, respectively which produced fast and sensitive catalytic reaction. H2O2 method detection carried out by LOD level of 0.12 ppm, and recovery parameters of 99%, in the linear range of 0.5-50 ppm. The mMy nanozyme was validated for H2O2 colorimetric detection. In conclusion an applicable way as a fast, sensitive, accurate and reproducible method has been suggested for occupational exposure monitoring. Performance of validated method is higher than international OSHAVI-6 method.