Preparation of fisetin loaded mesoporous silica nanocarrier to attenuate ischemia reperfusion injury

Fisetin, a naturally occurring flavonoid is effective in attenuating ischemia reperfusion injury (I/R) but due to its short half-life and poor water solubility, its clinical application is limited. In the present study, we determined the effect of fisetin loaded mesoporous nanoparticles on myocardial and renal I/R using cell line models. Chemically synthesized MSN, characterized by using SEM, TEM, FT-IR, BET, and zeta sizer. The BET surface area of MSN was calculated to be 501 m 2 /g where fisetin loaded MSN (F-MSN) exhibited 8.8% loading capacity and 88.7% encapsulation efficiency. The prepared F-MSN was biocompatible, possess better water solubility, low cytotoxicity (IC 50 value in H9C2: Fisetin-0.65 mg/ml, F-MSN-2.2 mg/ml, and IC 50 value in NRK52E: Fisetin-0.37 mg/ml, F-MSN-3.41 mg/ml) than pristine fisetin. Similarly, the cytoprotection of F-MSN against I/R was also higher than pristane fisetin in both cardiomyocytes (H9C2) and renal tubular cells (NRK52E). Graphical abstract