Effects of acute aerobic exercise on circulating sTLR and sRAGE profiles in normal- and abnormal-glucose-tolerant individuals

BMI-matched normal- (NGT, n = 10, 41 +/- 4y, 35.6 +/- 3.0 kg/m(2)) and abnormal-glucose-tolerant (AGT, n = 16, 51 +/- 3y, 34.3 +/- 1.5 kg/m(2)) participants were evaluated for body composition, metabolic health (oral glucose tolerance test [OGTT]), and VO(2)max. Participants also completed a treadmill walking test at 65% VO(2)max for 30 min. Total sRAGE, esRAGE, sTLR2, and sTLR4 were assessed via ELISA, and cRAGE was calculated. AGT exhibited greater (p < 0.05) body fat % (+24%), fasting plasma glucose (+37%), OGTT AUC (+59%), and HOMA-IR (+55%) and lower (p < 0.05) VO(2)max (-24%). sTLR2 was 33% lower in AGT than NGT (main effect, p = 0.034). However, sTLR2 did not change (p > 0.05) following AE. sTLR4 tended to be 36% lower in AGT than NGT (main effect, p = 0.096) and did not change following AE (p > 0.05). Total sRAGE and isoforms were similar (p > 0.05) between groups and did not change following AE (p > 0.05). sTLR2 was correlated with (p < 0.05) basal BG (r = -0.505) and OGTT AUC (r = -0.687). sTLR4 was correlated with basal BG (p < 0.10, r = -0.374) and OGTT AUC (p < 0.05, r = -0.402). Linear regressions were predictive of sTLRs in the basal state (sTLR2: R-2 = 0.641, p = 0.01; sTLR4: R-2 = 0.566, p = 0.037) and after acute exercise state (sTLR2: R-2 = 0.681, p = 0.004, sTLR4: R-2 = 0.568, p = 0.036).These findings show circulating sTLR profiles are disrupted in AGT and acute AE minimally modulates their levels.