Anticancer effect of superoxide dismutase on canine mammary gland tumour in vitro

BackgroundReactive oxygen species (ROS) have been shown to promote tumour growth and metastasis in human cell lines. The superoxide anion (center dot O2-) is produced during ROS formation and is involved in tumour cell signalling.ObjectivesSuperoxide dismutase (SOD) has been applied to canine mammary gland tumours to investigate its antitumour effects in vitro.MethodsCell proliferation, cell cycle cell migration assays, reverse transcription-quantitative polymerase chain reaction, and western blot analysis were performed to determine the effects of SOD on canine mammary tumour cell line.ResultsSOD treatment resulted in anti-proliferative effects and mediated cell cycle arrest in the canine mammary gland tumour cell lines (CIPp and CIPm). It also downregulated the expression of N-cadherin and Vimentin.ConclusionsThe results confirmed that SOD inhibits tumour cell proliferation and migration, thus supporting the potential applications of SOD as a chemotherapeutic agent for canine mammary gland tumours. SOD treatment showed anti-proliferative effects and mediated cell cycle arrest in the canine mammary gland tumour cell line (CIPp and CIPm). Our results show that SOD inhibits the proliferation and migration of tumour cells through downregulating the expression of N-cadherin and Vimentin activity. These findings support the potential application of SOD as a chemotherapeutic strategy for canine mammary gland tumours.image