Analyse humaner Hirnorganoide vom Millimeter- bis zum Nanometerbreich

We combine tissue expansion and light sheet fluorescence microscopy to analyze brain organoids. It is possible to zoom from a mesoscopic overview to super-resolution in a single imaging session, revealing cellular and subcellular structural details, such as the positions and spacing of pre- and postsynaptic proteins. Light sheet fluorescence expansion microscopy (LSFEM) greatly facilitates the qualitative and quantitative use of organoids in developmental and disease-related studies.