Accelerating Wound Healing: Unveiling Synergistic Effects of P25/SWCNT/Ag and P25/rGO/Ag Nanocomposites Within PRP-gelatin Scaffold, Highlighting the Synergistic Antimicrobial Activity.

Wound healing is a multifaceted biological process requiring innovative strategies to enhance efficiency and counter infections. In this groundbreaking study, we investigate the regenerative potential of platelet-rich plasma (PRP) integrated into a gelatin (GLT) scaffold along with nanocomposites of titanium dioxide (TiO2) (P25)/single-walled carbon nanotubes (SWCNTs)/Ag and P25/reduced graphene oxide (rGO)/Ag. Incorporating these advanced materials into the PRP/GLT delivery system aims to optimize the controlled release of growth factors (GFs) and leverage the exceptional properties of nanomaterials for enhanced tissue repair and wound healing outcomes. Antioxidant activity assessment using 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity reveals the superior performance of P25/SWCNTs/Ag compared to P25/rGO/Ag. Their synergistic effects are evaluated in conjunction with antibacterial and antifungal antibiotics. Furthermore, the wound healing potential of P25/SWCNTs/Ag and P25/rGO/Ag, combined with PRP/GLT, is examined. Notably, both nanocomposites exhibit promising synergistic effects with gentamicin and fluconazole against pathogenic strains. Significantly, the inclusion of non-activated PRP substantially augments the wound healing efficacy of P25/SWCNTs/Ag on days 3 (p < 0.01) and 15 (p < 0.05). These findings pave the way for advanced wound dressing and therapeutic interventions, capitalizing on the synergistic effects of PRP and nanomaterials, thus ultimately benefiting patients and advancing regenerative medicine.