Figure S8 from Antitumor Immune Responses in <i>B2M</i>-Deficient Cancers

Validation of YUMMER2.1 and YUMMER2.1 B2M-KO. (A) In vitro and in vivo growth curves in twenty-four clones from the YUMM2.1 UV cell line compared to WT. In vitro growth curves represent the percent confluence of cells (y-axis) over time (x-axis) as measured by IncuCyte continuous live-cell imaging. Error bars reflect the standard error of the mean across six replicates of each subline. YUMM2.1 UV clone 2 had similar proliferation rates and in vitro and in vivo growth curves compared to the parental cell line and was used for in vivo studies (YUMMER2.1). (B) Validation of B2M-KO by CRISPR/Cas9 gene editing from YUMMER2.1 using Western blot.