Identification of Cutibacterium acnes in spondylitis by metagenomics analysis

Research Square (Research Square)(2022)

引用 0|浏览8
暂无评分
摘要
Abstract Background: Identifying pathogens with culture-negative pyogenic spondylitis was difficult. Shotgun metagenomic sequencing is an unbiased and culture-free approach to the diagnosis of infectious diseases. There were, however, a variety of contamination factors that confounded the precision of metagenomic sequencing. Methods: This study proposed a contamination-robust protocol for clinical metagenomic sequencing. Using this protocol, we were able to identify Cutibactierum modestum in tissue specimen of infectious spondylitis. Results: The identification of Cutibactierum modestum and annotation of its resistome were annotated. The patient recovered fully. Conclusions: This application of next-generation sequencing using the contamination-robust protocol to assist the diagnosis of Cutibactierum modestum infection provides a new perspective in the clinical approach against spinal osteomyelitis and illustrates the potential of this technique in rapid etiological diagnosis.
更多
查看译文
关键词
cutibacterium acnes,spondylitis
AI 理解论文
溯源树
样例
生成溯源树,研究论文发展脉络
Chat Paper
正在生成论文摘要