Hsa-MiR-590-3p promotes the malignancy progression of pancreatic ductal carcinoma cell by inhibiting the expression of p27 and PPP2R2A via G1/S cell cycle pathway

Abstract Background : miR-590-3p plays an important role in the occurrence and development of many cancers, but its role in pancreatic cancer has not been reported. Objective : Investigate the effect and target genes of miR-590-3p in pancreatic cancer, so as to provide new diagnostic and therapeutic ideas for pancreatic cancer. Methods : qRT-PCR, MTT, clonal formation assay, flow cytometry and transwell assay were used to detect malignant cell behaviors. TargetScan (http://www.targetscan.org) was used to predict the binding region of miR-590-3p to target genes. Immunohistochemistry and Western blot were used to detect protein expression. Results : hsa-miR-590-3p expressed significantly higher in PC tissues than paired normal pancreas and its expression level was associated with PC tumor size (P=0.042) and preoperative CA19-9 level (P=0.046). Its overexpression promoted PC cell proliferation, invasion and migration following with the p27 and PPP2R2A protein down-regulation, and vice versa. Dual-luciferase reporter assay confirmed that p27 and PPP2R2A were direct target genes of miR-590-3p. Overexpression of P27 and PPP2R2A reversed the promoting effect of miR-590-3p on cell proliferation, migration and invasion. Conclusion : MiR-590-3p promote the development of pancreatic cancer by directly downregulated p27 and PPP2R2A via the G1/S cell cycle pathway.