Complete genome sequence of a novel mitovirus isolated from the fungus Fusarium oxysporum f. sp. ginseng causing ginseng root rot

Abstract A novel Mitoviridae virus, “Fusarium oxysporum mitovirus 2” (FoMV2), was isolated from the pathogenic Fusarium oxysporum f. sp. ginseng strain 0414 infesting Panax ginseng . The FoMV2 genome was 2388 nt in length and 30.57% in GC content. It carried an ORF encoding a 713-amino-acid RNA-dependent RNA polymerase (RdRp) with a molecular weight of 83.05 kDa. The homology between FoMV2 and the viruses isolated from “Botrytis cinerea mitovirus 8” and “Fusarium verticillioides mitovirus 1” was 87.94% and 77.85%, respectively. Phylogenetic analysis showed that FoMV2 belonged to Mitoviridae . To our knowledge, this is the first mycovirus isolated from F. oxysporum by ginseng root rot.