Comparison of malondialdehyde, hydroxynonenal, isoprostane and 8-hydroxydeoxyguanosine in osteoarthritis patients and healthy controls

Objective: To study the level of oxidative Stress markers like malondialdehyde, 4 Hydroxynonenal (HNE), Isoprostane and 8 hydroxyguanosine was measured in the serum, saliva and urine of the patients suffering from osteoarthritis. Methods: In this study, oxidative stress markers in the body fluids of Osteoarthritic( OA) patients were compared with the control groups. The level of stress markers were determined by commercially available quantitative ELISA kits. Result: Our findings indicate that the highest levels of these oxidative stress markers were detected in serum followed by urine. The saliva overall presented less levels of oxidative stress markers in individuals with and without OA. In addition to this detection of MDA in serum sample was found to be the most important tool in the detection of OA. Conclusion: the clinical significance of biomarkers of oxidative stress in humans must come from a critical analysis of the markers that should give an overall index of redox status in particular conditions. Keywords: Osteoarthritis, MDA, HNE, 8-Hydroxyguanosine, Isoprostane, lipidperoxidation