Validation of hemoglobin measurement in venous blood using HemoCue for the Ensanut 2022

Objective. To validate hemoglobin (Hb) concentration in venous blood (VB) using the HemoCue 201+ in a subsample of children and women from the Mexican National Health and Nutrition Survey 2022. Materials and methods. Prior to field work 50 HemoCue 201+ devices were verified using venous blood. During the field work 57 children (aged 1-11) and 62 women (aged 12-49) donated 3 mL of VB each. Hb was measured in each device of HemoCue 201+ and in a hematologic autoanalyzer for the validation. Results. No significant bias was found in most of the devices. An adjustment criterion was used for 22 devices. Hb mean difference results were -0.049 +/- 0.578 g/dL in children and -0.098 +/- 0.628 g/dL in women. Conclusions. The HemoCue 201+ is a valid tool for estimating Hb concentration to produce reliable estimates of anemia prevalence when using venous blood.