FgSdhC Paralog Confers Natural Resistance toward SDHI Fungicides in Fusarium graminearum
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY(2023)
摘要
Fusarium graminearum exhibited natural resistance to a majority of succinate dehydrogenase inhibitor fungicides (SDHIs) and the molecular mechanisms responsible for the natural resistance were still unknown. Succinate dehydrogenase subunit C (SdhC) is an essential gene for maintaining succinate-ubiquinone oxidoreductase (SQR) function in fungi. In F. graminearum, a paralog of FgSdhC named as FgSdhC(1) was identified. Based on RNA-Seq and qRT-PCR assay, we found that the expression level of FgSdhC(1) was very low but upregulated by SDHIs treatment. Based on reverse genetics, we demonstrated that FgSdhC(1) was an inessential gene in normal growth but was sufficient for maintaining SQR function and conferred natural resistance or reduced sensitivity toward SDHIs. Additionally, we found that the standard F. graminearum isolate PH-1 had high sensitivity to a majority of SDHIs. A single nucleotide variation (C to T) in the FgSdhC(1) of isolate PH-1, resulting in a premature termination codon (TAA) replacing the fourth amino acid glutamine (Q), led to the failure of FgSdhC(1) to perform functions of conferring nature resistance. These results established that a dispensable paralogous gene determined SDHIs resistance in natural populations of F. graminearum.
更多查看译文
关键词
Fusarium graminearum,succinate dehydrogenasesubunitC,paralog,natural resistance,SDHI,molecular functions
AI 理解论文
溯源树
样例
![](https://originalfileserver.aminer.cn/sys/aminer/pubs/mrt_preview.jpeg)
生成溯源树,研究论文发展脉络
Chat Paper
正在生成论文摘要