Physicochemical, rheological, antioxidant and immunological properties of four novel non-inulin (poly)saccharides from Asparagus cochinchinensis

The impacts of four extraction techniques, including hot water, ultrasonic-assisted, complex enzyme-assisted and acid-assisted methods, on the morphological, physicochemical properties and bioactivities of Asparagus cochin-chinensis (poly)saccharides (EACP, WACP, UACP, and AACP) were investigated and compared. The four samples were mainly composed of glucose, fructose, and galactose with molar ratios of 50.8:22.7:4.4 for WACP, 53.9:26.0:5.3 for UACP, 35.6:14.1:21.4 for AACP and 45.0:15.6:9.0 for EACP, respectively. The rheological result showed that ACPs were non-Newtonian fluids. EACP with high purity (97.65 %) had good DPPH center dot, O-2(center dot-) and ABTS(center dot+) radical scavenging activities, and significantly promoted the proliferation of the RAW264.7 cells at low concentration. UACP had good Fe2+ chelating ability, radical (DPPH center dot, O-2(center dot-) and OH center dot) scavenging activities, which might be attributed to the existence of triple-helix structure. AACP had high yield, molecular weight (17,477.2 Da), high crystallinity (23.33 %), and good radical (OH center dot and ABTS(center dot+)) scavenging activities. All four significantly stimulated the transcript expression levels of TNF-alpha, IL-1 beta and IL-6, as determined by RT-PCR. These results suggest that the exploitation and utilization of non-inulin (poly)saccharides extracted by ultrasonic-assisted, complex enzyme-assisted and acid-assisted extraction methods are potentially valuable as effective and natu-ral immune adjuvants and antioxidants.