Ionic Liquid Interface as a Cell Scaffold

ADVANCED MATERIALS(2024)

引用 0|浏览3
暂无评分
摘要
In sharp contrast to conventional solid/hydrogel platforms, water-immiscible liquids, such as perfluorocarbons and silicones, allow the adhesion of mammalian cells via protein nanolayers (PNLs) formed at the interface. However, fluorocarbons and silicones, which are typically used for liquid cell culture, possess only narrow ranges of physicochemical parameters and have not allowed for a wide variety of cell culturing environments. In this paper, it is proposed that water-immiscible ionic liquids (ILs) are a new family of liquid substrates with tunable physicochemical properties and high solvation capabilities. Tetraalkylphosphonium-based ILs are identified as non-cytotoxic ILs, whereon human mesenchymal stem cells are successfully cultured. By reducing the cation charge distribution, or ionicity, via alkyl chain elongation, the interface allows cell spreading with matured focal contacts. High-speed atomic force microscopy observations of the PNL formation process suggest that the cation charge distribution significantly altered the protein adsorption dynamics, which are associated with the degree of protein denaturation and the PNL mechanics. Moreover, by exploiting dissolution capability of ILs, an ion-gel cell scaffold is fabricated. This enables to further identify the significant contribution of bulk subphase mechanics to cellular mechanosensing in liquid-based culture scaffolds. Cell culturing at hydrophobic molecular liquids, such as fluorinated carbon and silicones, is attracting attention as a fundamental technology for highly efficient growth of useful cell resources. Here, it is attempted to utilize physicochemical property designable ionic liquid (IL) interface for cell culture, which provides a unique liquid | liquid interface that is highly polar but phase-separated from water. image
更多
查看译文
关键词
cell cultures,gels,ionic liquids,liquid interface,mechanobiology
AI 理解论文
溯源树
样例
生成溯源树,研究论文发展脉络
Chat Paper
正在生成论文摘要