Phyto-nano-MgO quantum dots by ultrasonic formulation for evaluation of toxin In-Vivo/Vitro/Silico sequels

Research on agrarian and communal pests through the use of biogenic 'phyto-MgO' nanoflakes and nanoflorets via ultrasonic waves as nano-biopesticides is marked as a key to success for green sustainable ventures. The combination of natural aqua -interlaced biocidal-floral extracts of Clitoria ternatea and Bauhinia variegata with Mg2+ salts formed a new nano -template in a quick, efficient, affordable, and environment -friendly way and served as benign bio-reducers and bio-stabilizers without cluster formation through agglomeration. UV-visible, Fourier transform -infrared (FT-IR), X-ray diffraction (XRD), high -resolution -transmission electron microscopy (HR-TEM), filed emission -scanning electron microscopy (FE-SEM), and energy dispersive X-ray (EDX) were used for analysis of the morphology of phyto-MgO_CT and MgO_BV quantum dots, while ultra -high performance liquid chromatography-high-resolution mass spectrometry (UHPLC-HRMS) and FT-IR spectroscopy were used to characterize biomolecules and functionals. In -vitro toxicities were: 1.33-24.43 and 1.27-41.71 mu g/mL with commendable half maximal effective concentration (EC50) values on the agrarian fungal and bacterial pathogens for MgO_CT and MgO_BV. In -vitro phyto-quantification revealed that 'phyto-MgO' is a strong anti -oxidant with alpha, alpha-diphenyl-beta-picrylhydrazyl (DPPH) inhibition of 95.00 % and half -maximal inhibitory concentration (IC50) of 1.0 mu g/mL. The MgO_CT had a better capacity compared to MgO_BV. In -vivo toxicities were 6.87-92.11 and 57.99-222.61 mu g/mL with low doses of 50 % lethal concentration (LC50) and 90 % lethal concentration (LC90) on mosquito -vectors (MV). In-silico computational docking of the receptor e -proteins of MV and e-ligand nano-MgO inferred electrostatic, C -H, Metal -O, and H -bond molecular interactions. The important findings suggested the use of a sustainable and eco-friendly module in a synergistic move to exterminate pests in a unique genre to protect environmental wealth.