From Short Circuit to Completed Circuit: Conductive Hydrogel Facilitating Oral Wound Healing

The primary challenges posed by oral mucosal diseases are their high incidence and the difficulty in managing symptoms. Inspired by the ability of bioelectricity to activate cells, accelerate metabolism, and enhance immunity, a conductive polyacrylamide/sodium alginate crosslinked hydrogel composite containing reduced graphene oxide (PAA-SA@rGO) is developed. This composite possesses antibacterial, anti-inflammatory, and antioxidant properties, serving as a bridge to turn the "short circuit" of the injured site into a "completed circuit," thereby prompting fibroblasts in proximity to the wound site to secrete growth factors and expedite tissue regeneration. Simultaneously, the PAA-SA@rGO hydrogel effectively seals wounds to form a barrier, exhibits antibacterial and anti-inflammatory properties, and prevents foreign bacterial invasion. As the electric field of the wound is rebuilt and repaired by the PAA-SA@rGO hydrogel, a 5 x 5 mm2 wound in the full-thickness buccal mucosa of rats can be expeditiously mended within mere 7 days. The theoretical calculations indicate that the PAA-SA@rGO hydrogel can aggregate and express SOX2, PITX1, and PITX2 at the wound site, which has a promoting effect on rapid wound healing. Importantly, this PAA-SA@rGO hydrogel has a fast curative effect and only needs to be applied for the first three days, which significantly improves patient satisfaction during treatment. In this work, they developed a conductive hydrogel (PAA-SA@rGO)with antibacterial, anti-inflammatory, and antioxidant properties, enhancingwound healing. It forms a protective barrier, stimulates fibroblast activity,and accelerates tissue regeneration. In rats, it rapidly repaired buccal mucosawounds within 7 days, requiring application only for the initial 3 days. Thehydrogel also upregulates SOX2, PITX1, and PITX2 mRNA, promoting geneexpression and ion diffusion in wounds. This study offers a new approach forbioelectric and bioactive electronic stimulation in wound healing, improvingpatient comfort. image