Biomimetic hydrogel based on HET-s amyloïd fibers for long-term culture of primary hippocampal neurons
arxiv(2024)
摘要
Historically, amyloid fibers (AF) in research has always been linked to
degenerative diseases. However, HET-s AF, by their morphology and function,
have only little in common to pathogenic amyloid fibers such as
α-synuclein or ae̱ṯa̱ and they have appeared as promising candidate
for biocoating since few years. Here we have shown than HET-s amyloid fibers
hydrogel is an extremely polyvalent coating material for the in vitro culture
of primary hippocampal neurons. First, the non-cytotoxicity was demonstrated in
vitro using standardized ISO protocols. Then, it is shown that in vitro culture
of primary hippocampal neurons on HET-s AF hydrogels could last more than 45
days with clear signatures of spontaneous network activity, with which is a
feat that not many other coatings have achieved yet. Finally, interactions
between the cells, the dendrites and the hydrogels are highlighted, showing
that dendrites might be able to penetrate the hydrogels in depth, therefore
allowing recordings even within micrometer-thick hydrogels. In the end, those
properties combined with group functionalization using standard biochemistry
techniques, makes HET-s hydrogels ideal candidates to be used for the long-term
growth of neurons as well as other types of cells. This versatility and
easiness to use are definitely still unheard, especially for protein material.
Due to its ability to transform from dry films to hydrogel when in contact with
the extracellular matrix (ECM), it could also be used for in vivo implants,
solving the issue of hydrogel damaging during the implant surgery.
更多查看译文
AI 理解论文
溯源树
样例
生成溯源树,研究论文发展脉络
Chat Paper
正在生成论文摘要