Living Antimicrobial Wound Dressings: Using Probiotic-Loaded, Alginate Nanofibers for Protection against Methicillin-Resistant Staphylococcus aureus

Living probiotic bacteria can be used as an alternative treatment to fight antibiotic-resistant, pathogenic bacteria. Electrospinning probiotics into nanofibers allows the probiotics to be conveniently applied like a wound dressing to protect open wounds while providing antimicrobial activity. In this letter, we encapsulated Lactococcus lactis into biocompatible, alginate-based nanofiber scaffolds. After cross-linking the scaffold to increase the chemical stability of the fibers, the encapsulated L. lactis cells maintained their ability to inhibit the growth of Staphylococcus aureus. This living wound dressing was especially effective at inhibiting the growth of clinically relevant methicillin-resistant S. aureus.