Preparation of aminated poly (L-lactic acid) film coated with multilayers of mussel-inspired heparin-like carboxymethylcellulose sodium and chitosan for anticoagulant materials

In this study, dopamine hydrochloride was grafted onto the sulfated carboxymethylcellulose sodium with different sulfated degree (DA-g-SCMCS) as heparin-like polysaccharides. By studying the preliminary structure and coagulation time of DA-g-SCMCS, the DA-g-SCMC2 possessing good anticoagulant properties were selected to be assembled layer by layer on the surface of aminated poly (L-lactic acid) (APLLA) film with chitosan (CS). The characterization properties including morphologies and wettability of DA-g-SCMC2/CS multilayers modified APLLA films were studied by scanning electron microscope and water contact angle instrument. Anticoagulation tests revealed that the (DA-g-SCMC2/CS)15 multilayers modified APLLA film experienced a decrease of 37.5% and 73.68% in the adsorption of bovine serum albumin (BSA) and fibrinogen (FBG), respectively. And the platelet adhesion ability was reduced by 92.42% compared with the APLLA film. The degree of platelet activation (PF4 and TAT) and hemolysis rates of modified APLLA films also decreased significantly. Besides, the modified APLLA films promoted the growth of L929 cells and exhibited remarkable anti-inflammatory and antibacterial abilities. To summarize, the APLLA films modified with heparin-like multifunctional DA-g-SCMC2/CS multilayers exhibit outstanding biocompatibility, cytocompatibility, anti-inflammatory, and antibacterial characteristics, showcasing their potential as anticoagulation materials. Preparation process of DA-g-SCMC/CS multilayers modified APLLA film with anticoagulant function.image