Anti-Biofilm Activities of Silver Nanoparticle Conjugated Rhazya stricta Phytocompounds in Periprosthetic Joint Infections by Staphylococcus aureus

Background and Objective: Periprosthetic Joint Infections (PJIs) caused by multi-drug resistant biofilm-forming Staphylococcus aureus make the treatment challenging. The present study makes an effort to find out the effect of AgNP- Rhazya stricta extract conjugate on biofilms associated with PJIs in total hip arthroplasties (THAs). Materials and Methods: The strains of S. aureus were identified and analyzed for their antibiogram against the antibiotics selected. The biofilm-forming abilities of the isolated strains were determined using the crystal violet method and the effect of green synthesized AgNP- Rhazya stricta extract conjugate coated on prosthetic femur head was analyzed using confocal microscopy. Results: Two isolates were identified as S. aureus and they showed resistance to all of the antibiotics tested and also have shown the ability to form biofilms. The AgNP-Rhazya stricta extract conjugate showed better antibacterial activity against O5 strain of Staphylococcus aureus compared with O3. The AgNP-Rhazya stricta extract-coated prosthetic femur heads were found to be reducing the biofilms on them. Conclusion: As the thickness of biofilms formed by MDR S. aureus was reduced on AgNP- Rhazya stricta extract conjugate coated metallic femoral head. Further studies are required on the AgNP- Rhazya stricta extract to make it available for its clinical use.