Reshaping the Substrate Binding Pocket of -Amino Acid Dehydrogenase for the Synthesis of Aromatic -Amino Acids

By reshaping the substrate-binding pocket of beta-amino acid dehydrogenase (beta-AADH), some variants were obtained with up to 2560-fold enhanced activity toward the model substrates (S)-beta-homophenylalanine and (R)-beta-phenylalanine. A few aromatic beta-amino acids were prepared with >99% ee and high isolated yields via either kinetic resolution of racemates or reductive amination of the corresponding beta-keto acids. This work expands the catalytic capability of beta-AADHs and highlights their practical application in the synthesis of pharmaceutically relevant beta-amino acids.