Pseudidiomarina fusca sp. nov., Isolated from the Surface Seawater of the Western Pacific Ocean

The Gram-negative marine bacterium GXY010(T), which has been isolated from the surface seawater of the western Pacific Ocean, is aerobic, non-motile and non-flagellated. Strain GXY010(T) exhibits growth across a temperature range of 10-42 degree celsius (optimal at 37 degree celsius), pH tolerance from 7.0 to 11.0 (optimal at 7.5) and a NaCl concentration ranging from 1.0 to 15.0% (w/v, optimal at 5.0%). Ubiquinone-8 (Q-8) was the predominant isoprenoid quinone in strain GXY010(T). The dominant fatty acids (>10%) of strain GXY010(T) were iso-C15:0 (14.65%), summed feature 9 (iso-C-17:1 omega 9c and/or 10-methyl C16:0) (12.41%), iso-C-17:0 (10.85%) and summed feature 3 (C-16:1 omega 7c and/or C-16:1 omega 6c) (10.41%). Phosphatidylethanolamine (PE), phosphatidylglycerol (PG), diphosphatidylglycerol (DPG), unidentifiable glycolipid (GL) and four non-identifiable aminolipids (AL1-AL4) were the predominant polar lipids of strain GXY010(T). The genomic DNA G+C content was identified as a result of 48.0% for strain GXY010T. The strain GXY010(T) genome consisted of 2,766,857 bp, with 2664 Open Reading Frames (ORFs), including 2586 Coding sequences (CDSs) and 78 RNAs. Strain GXY010(T) showed Average Nucleotide Identity (ANI) values of 73.4% and 70.6% and DNA-DNA hybridization (DDH) values of 19.2% and 14.5% with reference species Pseudidiomarina tainanensis MCCC 1A02633(T) (=PIN1(T)) and Pseudidiomarina taiwanensis MCCC 1A00163(T) (=PIT1T). From the results of the polyphasic analysis, a newly named species, Pseudidiomarina fusca sp. nov. within the genus Pseudidiomarina, was proposed. The type strain of Pseudidiomarina fusca is GXY010(T) (=JCM 35760(T) = MCCC M28199(T) = KCTC 92693(T)).