The recombinant l-lysine -oxidase from the fungus Trichoderma harzianum promotes apoptosis and necrosis of leukemia CD34+hematopoietic cells

BackgroundIn hematologic cancers, including leukemia, cells depend on amino acids for rapid growth. Anti-metabolites that prevent their synthesis or promote their degradation are considered potential cancer treatment agents. Amino acid deprivation triggers proliferation inhibition, autophagy, and programmed cell death. l-lysine, an essential amino acid, is required for tumor growth and has been investigated for its potential as a target for cancer treatment. l-lysine alpha-oxidase, a flavoenzyme that degrades l-lysine, has been studied for its ability to induce apoptosis and prevent cancer cell proliferation. In this study, we describe the use of l-lysine alpha-oxidase (LO) from the filamentous fungus Trichoderma harzianum for cancer treatment. ResultsThe study identified and characterized a novel LO from T. harzianum and demonstrated that the recombinant protein (rLO) has potent and selective cytotoxic effects on leukemic cells by triggering the apoptotic cascade through mitochondrial dysfunction. ConclusionsThe results support future translational studies using the recombinant LO as a potential drug for the treatment of leukemia.