Enhancing short-chain fatty acids production via acidogenic fermentation of municipal sewage sludge: Effect of sludge characteristics and peroxydisulfate pre-oxidation

This study first employed a combined pretreatment of low-dose peroxy-disulfate (PDS) and initial pH 10 to promote short-chain fatty acids (SCFAs) production via acidogenic fermentation using different types of sewage sludge as substrates. The experimental results showed that the yield of maximal SCFAs and acetate proportion after the combined pretreatment were 1513.82 +/- 28.25 mg chemical oxygen demand (COD)/L and 53.64%, and promoted by 1.28 and 1.56 times higher, respectively, compared to the sole initial pH 10 pretreatment. Furthermore, in terms of the disintegration degree of sewage sludge, it increased by more than 18% with the combined pretreatment compared to the pretreatment of sole initial pH 10. Waste-activated sludge (WAS) from A2/O and Bardenpho processes were more biodegradable, explained by the 1.47- and 1.35-times higher disintegration rate than those from oxidation ditch and they favored acetate dominant fermentation. Correlation analysis revealed a strong correlation (p <= 0.01) between SCFAs production and soluble COD, total proteins, proteins in soluble-extracellular polymeric substances (SEPS), total polysaccharides, and polysaccharides in SEPS. Mechanism explorations showed that preoxidation with PDS enhanced the solubilization and biodegradability of complex substrates, and altered the microbial community structure during the fermentation process. Firmicutes and Tetrasphaera were proven to play a key role in improving SCFA production, especially in promoting acetate production by converting additional SCFAs into acetate. Additionally, the addition of PDS greatly promoted sulfur and iron-related metabolic activities. Finally, the combined pretreatment was estimated to be a cost-effective solution for reutilizing and treating Fe-sludge. Short-chain fatty acids (SCFAs) production via acidogenic fermentation was largely enhanced by a combination pretreatment of low-dose peroxydisulfate (PDS) coupling with initial pH 10. Mechanism explorations showed that PDS pre-oxidation not only promoted the solubilization and biodegradability of complex substrate, but also modified the microbial community structure throughout the fermentation process. image