FIGURE 4 from Targeting Tryptophan Catabolism in Ovarian Cancer to Attenuate Macrophage Infiltration and PD-L1 Expression

A dual IDO1/TDO2 inhibitor reduces macrophage tumor infiltration. A, Murine ID8 tumor study design. Representative ID8 tumor images (B), MRI (dotted lines; C), and tumor weight (n = 7–9/group; D). E, ID8 tumor volume. F, KYN levels in mouse serum. Blinded binning of T cells (CD3⁺; G) and macrophage (F4/80+; H) infiltration from ID8 tumors. I, mIHC panel used on ID8 mouse tumors. J, mIHC analysis of mouse ID8 tumors. K, In the human ovarian cancer TCGA dataset, TIMERv2 analysis of TDO2 and “M2” protumor macrophage infiltration. L, mIHC analysis of primary HGSC human tumors. Representative images. Quantification of tumor-associated cytotoxic T cells (M), Tregs (N), and macrophages (O) based on IL6 status of primary HGSC human tumors. Error bars, SEM. Statistical test, unpaired t test and unpaired t test with Welch correction (M–O). *, P < 0.05; **, P < 0.01; ****, P < 0.0001