Electrolyte Leakage Assay to Analyze Membrane Integrity in Leaves v1

The production of reactive oxygen species occurs naturally in certain cellular organelles as metabolic byproducts. However, under stress conditions, their accumulation increases, resulting in cell death and the leakage of intracellular electrolytes. Consequently, quantifying electrolyte leakage has been widely accepted as an indicator of membrane integrity. This protocol delineates specific steps for measuring electrolyte leakage from plant tissues, providing insights into cellular damage caused by various stressors, including biotic, abiotic, and other factors.