Research Progress of Three-dimensional Fluorescence Coupled with Chemical Multiway Calibration

Fluorescent technology is widely used in many fields due to its high sensitivity. However, the direct quantification of one target analyte in complex system is still difficult to be achieved when using the traditional fluorescent method without any pretreatment separation procedure. This is due to the fact that serious overlapping of fluorescence spectra often occurs, mainly originating from natural interferences in complex sample backgrounds, or the interferents with similar structures to a target analyte, particularly in the simultaneous analysis of multi. components samples. The rapid development of modern analytical instruments and three. way data collection techniques has led to a resurgence of interest in the development of chemomet. rics. based analytical strategies, which might light a new avenue to simple experimentation using "mathematical separation. as a replacement or enhancement of "physical or chemical separation. of uncalibrated background or interferents. These methods can offer a highly attractive property, called "second. order advantage", which allows for the direct and rapid determination of a single target component or simultaneous determination of multiple target components in complex samples, even in the presence of uncalibrated interferences. The property has been a hotspot in the current chemometric domain, and was successfully employed for many applications, such as pharmaceuticals, biological, food, environmental analysis and so on.