FIGURE 4 from Identification of Enhanced Vaccine Mimotopes for the p15E Murine Cancer Antigen

Efficacy of p15E-3C and p15E-3M e-mimotope vaccines at submicrogram peptide doses in prophylactic and therapeutic MC38 tumor challenges. Mice were immunized intramuscularly with CPQ liposomes containing 0.8 µg PHAD, 0.8 µg QS-21, and 0.5 µg p15E wild-type, p15-3C, or p15-3M on days 0 and 7 and were challenged with 1 × 10⁶ MC-38 cells subcutaneously on day 14 (n = 5 mice per group). Individual tumor volumes (A) and the percentage of mice with tumor length under 10 mm (B). For therapeutic challenge, mice were inoculated with 1 × 10⁵ MC-38 cells on day 0 and immunized with 0.5 µg p15E wild-type or 3C or 3M liposomal vaccine on day 2 and 9 (n = 5 mice per group). Individual tumor volumes (C) and the percentage of mice with a tumor <10 mm in diameter (D). Statistical analysis by log-rank (Mantel–Cox) test. * and ** indicates P < 0.05 and 0.01, respectively.