miR-29c Carried by Lipid Nanoparticles Mediates TGF- Signaling Pathway in Renal Fibrosis

miR-29c is related to renal fibrosis. Lipid nanoparticles can inhibit cell growth. This study mainly explores whether miR-29c carried by lipid nanoparticles may regulate the expression of TGF-beta signaling and then involves in renal fibrosis. Kidney fibrosis cells HK-2 were intervened with 20 mu mol/ L miR-29c carried by lipid nanoparticles followed by analysis of the proliferation number and cell cycle changes of HK-2 cells, expression of TGF-beta pathway protein, and relationship between TGF-beta and miR-29c. Mice were infused with Ang II (1000 ng/kg/min) for 4 weeks to establish a mouse model of renal fibrosis. After treatment with miR-29c carried by lipid nanoparticles and PBS, the changes of renal fibrosis and the expression of TGF-beta were measured. The higher the concentration of miR-29c carried by lipid nanoparticles, the more significant the decrease in cell proliferation, and cells in S phase began to decline significantly (P < 0.05). Cell number in lipid nanoparticle+ PBS group was the lowest and cells in PBS group and lipid nanoparticle+ TGF-beta inhibitor group were higher. TGF-beta is a target gene of miR-29c. When the concentration of miR-29c in lipid nanoemulsion was 20 mu mol/L, the expression of TGF-beta protein decreased. miR-29c-carried lipid nanoparticles significantly attenuated Ang II-induced kidney injury. TGF-beta was highly expressed in renal fibrosis compared with control mice and the expression of TGF-beta was decreased after lipid nanoparticle treatment. miR-29c carried by lipid nanoparticles can inhibit the proliferation of renal fibrosis cells, regulate the TGF-beta pathway, and ultimately control abnormal cell proliferation.