Assessment of genetic homogeneity of in-vitro propagated apple root stock MM 104 using ISSR and SCoT primers

Vandana Bisht, Janhvi Mishra Rawat,Kailash Singh Gaira,Sumit Purohit, Jigisha Anand, Somya Sinha, Debasis Mitra,Farid S. Ataya,Ahmed M. Elgazzar,Gaber El-Saber Batiha,Balwant Rawat

BMC Plant Biology(2024)

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摘要
Apple is an important fruit crop that is always in demand due to its commercial and nutraceutical value. Also, the requirement for quality planting material for this fruit crop for new plantations is increasing continuously. In-vitro propagation is an alternative approach, which may help to produce genetically identical high grade planting material. In this study, for the first time, an efficient and reproducible propagation protocol has been established for apple root stock MM 104 via axillary bud. Culturing axillary buds on Murashige and Skoog apple rootstock (MM 104) resulted in better in-vitro propagation. (MS) basal medium supplemented with 3.0
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关键词
Fruit crop,Micro propagation,Molecular markers,Inter simple sequence repeats,Start Codon targeted,Genetic stability
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