Quantification of neurofilament light and glial fibrillary acidic protein in finger-prick blood

Abstract An accurate diagnosis of neurodegenerative disease and traumatic brain injury is important for prognostication and treatment. Neurofilament light and glial fibrillary acidic protein are leading biomarkers for neurodegeneration and glial activation that are detectable in blood. Yet, current recommendations require rapid centrifugation and ultra-low temperature storage post-venepuncture. Here, we investigated if these markers can be accurately measured in finger-prick blood using dried plasma spot cards. Fifty patients (46 with dementia; 4 with traumatic brain injury) and 19 healthy volunteers underwent finger-prick and venous sampling using dried plasma spot cards and aligned plasma sampling. Neurofilament light and glial fibrillary acidic protein were quantified using a Single molecule array assay and correlations between plasma and dried plasma spot cards assessed. Biomarker concentrations in plasma and finger-prick dried plasma spot samples were significantly positively correlated (neurofilament light ρ=0.57; glial fibrillary acidic protein ρ=0.58, P<0.001). Finger-prick neurofilament light and glial fibrillary acidic protein were significantly elevated after acute traumatic brain injury with non-significant group-level increases in dementia (91% having Alzheimer’s disease dementia). In conclusion, we present preliminary evidence that quantifying glial fibrillary acidic protein and neurofilament light using finger-prick blood collection is viable, with samples stored at room temperature using dried plasma spot cards. This has potential to expand and promote equitable testing access, including in settings where trained personnel are unavailable to perform venepuncture.