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Effect of Desulfation of Polysaccharides from Sea Urchin Eggs Paracentrotus Lividus on Antiproliferative Activity

Chemistry Africa(2024)

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摘要
This study aims to study the impact of reducing the sulfate content and molecular weight of polysaccharides extracted from sea urchin eggs (Paracentrotus lividus) on antiproliferative activity against Caco-2 and B16 cell lines. Sulfated polysaccharides were initially extracted from sea urchin eggs, followed by a desulfation process. The characterization involved various techniques: Fourier-transform infrared spectroscopy for demonstrating desulfation, Gas Chromatography/Mass Spectrometry for determining monosaccharide composition, Size Exclusion Chromatography for confirming molecular weight reduction, and centesimal analysis for quantifying sulfate content. The initial molecular weight was 24,000 kg/mol decreased to 960 kg/mol, and sulfate content reduced from 9.41 to 1.31
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关键词
Paracentrotus lividus,Desulfated polysaccharides,Physico-chemical characterization,Antiproliferative activity
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