Biology, phylogenetic and evolutionary relations of Tradescantia mild mosaic virus isolates from Hungary

In Western Transdanubia in 2018 and in Central-Hungary in 2022 spiderwort plants showed flower breaking symptoms and mild mosaic on the foliage, which indicated a potential virus infection. One gram of symptomatic leaf sample was collected at both locations. Potyvirus-specific ELISA tests demonstrated potyvirus infection. To identify the virus species, potyvirus-specific RT-PCR was carried out on the samples. In both samples specific PCR products were detected and cloned into pGEM®-T Easy vector. The nucleotide sequences of the inserts were determined by Sanger sequencing. BLASTn searches on the complete coat protein region of both isolates demonstrated more than 99.87