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Guanidine acetic acid(GAA)is an amino acid derivative and an important precursor of creatine(Cr)and creatine phosphate(PCr).Cr plays an important role in animal production for body growth and health,but it has problems of instability and high cost in the actual production process.GAA is an effective alternative to Cr supplements.As a new type of nutritional additive,GAA has the functions of regulating energy metabolism,improving antioxidant function,regulating glycolysis,improving meat quality,saving arginine,and regulating blood sugar.In this paper,the physicochemical properties,anabolic pathways and biological functions of GAA were described,and the research results of GAA in pig,ruminant,poultry,and aquatic animal production were reviewed,in order to provide reference for further application of GAA in animal production.