Taenia solium is a tapeworm of the family Taeniidae that causes neurocysticercosis, a serious zoonotic disease in humans. Its life cycle involves pigs and wild boars as intermediate hosts and humans as the sole definitive host. Since poor sanitation and free-roaming pigs contribute to maintaining its life cycle, cysticercosis is endemic in developing countries across Asia, Africa, and Latin America, while local transmission is generally absent in developed countries. However, we unexpectedly identified three cases of cysticercosis in wild boars in Japan between 2014 and 2023. Genetic analyses were performed on six cysticerci collected from two wild boars independently captured in 2023. Phylogenetic analysis using three nuclear DNA markers confirmed that the cysticerci were indeed T. solium. Mitogenome sequencing from these cysticerci yielded six complete mitogenomes, each 13,712 bp in length and identical to each other. Haplotype network analysis using mitochondrial cox1 and cob sequences revealed that the cysticerci in Japan possess a haplotype distinct from haplogroups in other endemic regions, i.e., haplogroups in Asia, Africa/America, and Bhutan, indicating that the T. solium population is divided into at least four haplogroups. Subsequent phylogenetic inference from the mitochondrial 12 protein-coding genes demonstrated that the Japanese haplotype diverged from both the Asian and African/American haplogroups before the divergence of these two major haplogroups in the Early to Middle Pleistocene. Our findings indicate that the T. solium life cycle can be maintained in regions generally considered non-endemic, highlighting an overlooked risk of local transmission in developed countries. Furthermore, the genetic diversity and phylogenetic relationships revealed in this study may provide evidence for revisiting the "Out of Africa" hypothesis for T. solium. Comprehensive mitogenomic analyses based on additional specimens would hold the key to unraveling the evolutionary history of this tapeworm, which currently uses humans as its sole definitive host.
Climate change exerts a fundamental influence on the quality of the forage base and the safety of feed in Hungary, with the negative effects of interrelated climatic factors, in part due to their unpredictability, proving challenging to mitigate in practice. In order to maintain the health status of dairy herds and sustain the expected production level, it is essential to provide silage with a nutrient content adapted to the given lactation stage, high digestibility, proper fermentation quality, and stability after opening. Ensiling is a multi-step process requiring strict technological discipline, and the quality of the ensiled feed is influenced by the species and variety of forage crops, their cultivation techniques, and the ensiling technology itself. After the ensiling process, upon silo opening, aerobic deterioration processes may occur, which can negatively affect even well-fermented forage quality. In light of these factors, there is an increasing need to understand the factors influencing the aerobic deterioration of silage. Monitoring the aerobic stability characteristics of feed is crucial for assessing the quality, shelf life, and feed ability of fermented forages. This summary study reviews the microbiological and closely related biochemical factors influencing aerobic stability, as well as the effects of ensiling technology and environmental parameters on the aerobic deterioration of fermented feed.
Eight Eimeria spp. (Apicomplexa: Eimeriidae) have been isolated from the ring-necked pheasant (Phasianus colchicus Linnaeus), native to the temperate zone of Asia and eastern regions of Europe. Enteric coccidiosis has become a major issue associated with the breeding of farmed pheasants for game bird release or meat production. In this study, 35 fecal samples were collected from two-to-three-month-old ring-necked pheasants from four pheasant-rearing farms in Ehime Prefecture, Japan. Microscopic examination using a saturated sugar solution technique detected numerous subspherical oocysts from the samples of one farm and ellipsoidal Eimeria phasiani Tyzzer, 1929 oocysts from the three other farms. The subspherical oocysts were artificially sporulated and measured 18.6 µm by 15.7 µm with a 1.18 shape index (n = 150). Each oocyst contained four 10.7 µm × 5.8 µm sporocysts (n = 30) and one coarse refractile polar granule; no micropyle or residua were detected. Each sporocysts contained two sporozoites with one large and one small refractile body and sparsely distributed residua. The complete, 1,443-bp cytochrome c oxidase subunit I gene (cox1) of this isolate exhibited low sequence identity with published Eimeria spp. sequences including E. phasiani that was previously recorded in the same area. Meanwhile, the oocyst morphology most closely resembled that of Eimeria tetartooimia Wacha, 1973, but with distinct refractile polar granules and sporocyst residua. The available GenBank cox1 sequence of E. tetartooimia exhibited a sequence identity of < 94.5
